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Colonies were maintained in flow through conditions aboard the vessel and were shaded from direct sunlight.
Fish were maintained in flow through tanks under ambient conditions of light (photoperiod 10L/14D) and temperature (15 ± 2°C).
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All striped bass were maintained in flow-through tank systems receiving ambient Chesapeake Bay water.
CCA were collected together with corals at both sites and maintained in flow-through seawater tables.
Tripneustes gratilla, collected near Coffs Harbour, New South Wales (30°12.5'S. 153°16.1'E)., were maintained in flow-through aquaria (∼3500 L) at ambient temperature (∼24°C).
These colonies were maintained in flow-through outdoor aquaria (27°C) at the Australian Institute of Marine Science (Townsville, Australia) until coral spawning on the 29th (A. tenuis) and 30th (A. millepora) of October 2007.
They were maintained in flow-through freshwater (15°C) holding tanks under a 12 h:12 h L D light cycle.
During this period, fish were maintained in flow-through tanks containing 150 L of seawater (19 21°C, pH 7.8) under a 12/12-hr 12/12-hrrk photoperiod and fed dailight/darkg body weight) with commercial food (Morubel, Milan, Italy) corresphotoperiod2% of biomand in the tank.
Grouper juveniles were reared in 250-l circular fiberglass tanks maintained in a flow-through seawater system.
The fish were stabilized on a holding device, using a pair of orbital ridge acrylic clamps, wrapped in a wet Kim-Wipe and maintained in a flow-through system with perfusion tubes into their mouth by gill perfusion at 400 ml/min (Fig. 3).
The animals were immediately returned to the laboratory where they were maintained in a through-flow aquarium with a temperature of 0.6 ± 0.3°C, under a simulated natural light:dark cycle.
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