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Colonies were maintained in flow through conditions aboard the vessel and were shaded from direct sunlight.
Fish were maintained in flow through tanks under ambient conditions of light (photoperiod 10L/14D) and temperature (15 ± 2°C).
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All striped bass were maintained in flow-through tank systems receiving ambient Chesapeake Bay water.
CCA were collected together with corals at both sites and maintained in flow-through seawater tables.
Tripneustes gratilla, collected near Coffs Harbour, New South Wales (30°12.5'S. 153°16.1'E)., were maintained in flow-through aquaria (∼3500 L) at ambient temperature (∼24°C).
These colonies were maintained in flow-through outdoor aquaria (27°C) at the Australian Institute of Marine Science (Townsville, Australia) until coral spawning on the 29th (A. tenuis) and 30th (A. millepora) of October 2007.
They were maintained in flow-through freshwater (15°C) holding tanks under a 12 h:12 h L D light cycle.
During this period, fish were maintained in flow-through tanks containing 150 L of seawater (19 21°C, pH 7.8) under a 12/12-hr 12/12-hrrk photoperiod and fed dailight/darkg body weight) with commercial food (Morubel, Milan, Italy) corresphotoperiod2% of biomand in the tank.
Cells were maintained in flowing Krebs' solution at 20 22 °C containing (in mM) 120 NaCl, 3 KCl, 1.5 MgCl2.52.5 CaCl2, 10 HEPES, and 11.1 glucose (pH-adjusted to 7.3 with NaOH).
The mice were housed and maintained in laminar flow cabinets under specific pathogen-free conditions and throughout the entirety of the study, all efforts were made to minimize suffering.
High voltage potential maintained in a flow chamber can pasteurize juice at only moderately elevated temperatures.
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