Sentence examples for maintained in flasks from inspiring English sources

Ninth-passage HLEC derived from the same batch, as documented elsewhere [33], were maintained in flasks at 37°C with 5% CO2 in Medium 199 containing 10% (vol/vol) fetal calf serum, 50 units/ml penicillin-streptomycin and 0.25 mg of Fungizone (InVitrogen, Cergy Pontoise, France).

Meanwhile, cultures of 5 litters were maintained in flasks at similar condition with aeration.

PC12 cells (ATCC) were maintained in flasks in F12 medium (Invitrogen) supplemented with 15% horse serum (ATCC) and 2.5% Fetal bovine Serum (Invitrogen).

Briefly, monolayers of J774A.1 macrophages were maintained in flasks in a RPMI-1640 medium supplemented with 100  μg/mL gentamicin, 2 mM L-glutamine, and 10% heat-inactivated fetal bovine serum (FBS), in humidified air with 5% CO2 at 37°C.

L1210 cells (murine lymphoblastic leukaemia) were maintained in flasks at between 0.1 and 3×10 cells ml−1 in exponential phase growth (generation time ∼15 h) in RPMI 1640 medium (Life Technologies, Paisley, UK) at 37°C in a humidified atmosphere of 5% CO2.

Cells were maintained in flasks in RPMI-1640 with 10% fetal bovine serum (FBS), 100  μg/mL penicillin, and 100  μg/mL streptomycin, at 37°C in a humidified atmosphere of 5% CO2. 5 × 10 cells/well of EC-1 and ECa-109 cells were seeded in 96-well plates for 24 h before exposure to drug treatment.

The cultures were maintained in plastic flasks and incubated in CO2/air (5:95, v/v) at 37°C in a humidified incubator.

Cells were maintained in 175 cm2 flasks in at 37°C in 5% CO2 95% atmosphere humidified incubator and grown to sub-confluent monolayers before being detached using trypsin for use in animal models.

Cells were maintained in culture flasks at 37 °C in 5% CO2 atmosphere (Sanyo MCO-20AIC, Osaka, Japan).

MDA-MB-231 cells were maintained in sealed flasks with L-15 medium at 37°C.

When indicated, replicate cultures were maintained in nephelo flasks for estimation of culture growth by the settled cell volume.