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Oral fibroblasts were maintained in fibroblast growth medium.
Cultures were maintained in fibroblast media and passed every 6 days.
HDF cells were maintained in fibroblast growth medium (Cell Applications) supplemented with a low-serum-growth supplement.
Human adult dermal fibroblasts were obtained from TCS Cellworks and maintained in fibroblast growth medium (TCS Cellworks) with the same supplements as used in the HUVEC media.
Normal human primary dermal fibroblasts (NHDF) were purchased from Lonza (Walkersville, MD) and maintained in fibroblast growth medium-2 (FGM-2) BulletKit CC-31322, Lonza) in a humidified 5% CO2 incubator at 37°C.
Primary normal human dermal fibroblasts (NHDF) expressing GFP were temperature sensitive immortalized by protocols from Sirion Biotech GmbH (GE) and were maintained in Fibroblast growth medium (Promocell, GE) supplemented with 0.4 mg/ml G418.
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Of further interest is that flexibility in potential for chemokine production is maintained in fibroblasts derived from SSc skin.
Furthermore, this in vivo characteristic is maintained in the fibroblast for 50 passages as indicated by their continued resistance to H2O2.
In these studies we focus upon the role of ETC generated ROS on determination of the levels of (SH) 2Trx-ASK1 complex and activation of the p38 MAPK pathway in fibroblasts from young (3-4 middlemiddle aged 10-122 mos) and old (21-24 mos) wild type and Ames dwarf mice and whether this in vivo redox sensitive regulatory process is maintained in the fibroblast cell cultures.
In contrast to the frequent KSHV genome loss observed from Kaposi's Sarcoma spindle cells [51], MuHV-4 episomes were maintained in transformed fibroblasts for at least 3 weeks without evidence of viral lytic gene expression.
Whether they are maintained in cultured fibroblasts will also need to be determined.
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