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Animals used in this study were maintained in facilities in accordance with current regulations and observing 'Principles and Guidelines for the Use of Animals in Research' Issued by the French government according to the European community rules (Workman, 1998).
Mice were provided pelleted rodent diet (ProLab 3000; Purina Mills, Gray Summit, MO) and filtered water ad libitum and were maintained in facilities accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International.
Animals were housed in pathogen-free conditions and maintained in facilities of the University of Michigan Comprehensive Cancer Center and this study was included in our animal use protocol, approved by the University of Michigan University Committee on Use and Care of Animals (UCUCA).
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All mice were maintained in animal facilities according to animal care guidelines.
BALB/c mice were obtained from Charles River (Margate, UK) and bred and maintained in local facilities.
A register for recording of autoclaving was maintained in most facilities.
Female C57BL/6 J mice (Harlan) were bred and maintained in local facilities.
BALB/c female mice (10 12 weeks old) were maintained in the facilities of the University of Granada.
All mice were maintained in animal facilities under pathogen-free conditions.
BALB/c mice were originally obtained from Charles River (Margate, United Kingdom) and bred and maintained in local facilities.
Mice were maintained in our facilities and used for experimentation between 6 and 8 weeks of age.
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