Sentence examples for maintained in essential from inspiring English sources

In addition, live feeder free iPSC cultures maintained in Essential 8 media on Matrigel iPSCs from C4955 (passage 15 + 7) were provided to Applied Stem Cell Inc. (Menlo Park, CA) for teratoma analysis as previously described (Chen et al., 2012).

NKX2-5 eGFP/w hiPSCs (R.P.D. and C.L.M., unpublished data) were maintained in Essential 8 medium (Life Technologies) and differentiated as previously described (van den Berg et al., 2015).

The mouse neuroblastoma cell-line Neuro2A was maintained in Minimum Essential Media, supplemented with 1% non-essential amino acids, 10% fetal bovine serum, 100 U/ml penicillin and 100 U/ml streptomycin.

The cells were maintained in minimal essential medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum, 1% non-essential amino acids and an antibiotic-antimycotic mixture.

Cervical carcinoma (HeLa) cells were maintained in Minimal Essential Medium (MEM), 10% FBS supplemented with 2 mM glutamine and 0.1 mM non-essential amino acids.

D283-MED, T98G and U87MG cells were purchased from ATCC (Manassas, VA, USA) and were maintained in minimal essential medium with Earle's salts supplemented with 10% fetal calf serum, 1% non-essential amino acids and 1 mM sodium pyruvate.

J82 and Hela cells were maintained in minimum essential medium with 10% fetal bovine serum (FBS).

The neutralization assays were performed using Vero-E6 cells obtained from ATCC and maintained in minimal essential medium (MEM) (ThermoFisher Scientific) supplemented by 10% FBS (HyClone) and 1% penicillin-streptomycin at 5% CO2, 37 °C.

The cells were maintained in Minimal Essential Medium supplemented with 10% FBS, penicillin (100 U/ml), and streptomycin (100 μg/ml) in a humidified atmosphere of 50 μg/ml CO2 at 37 °C.

Daoy cells were propagated in Dulbecco's modified Eagle's medium (DMEM), D283 cells were maintained in minimum essential medium (MEM), both supplemented with 10%% fetal bovine serum, 100 units/mL penicillin G sodium and 100 μg/mL streptomycin (HyClone).

Rat basophilic leukemia (RBL) cells (RBL-2H3; ATCC number CRL-2256) were maintained in minimal essential medium (MEM) supplemented with 10%% FBS, 1 % Pen-Strep, and 1 % l-glutamine (L-glut).