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Cells were maintained in equal parts Leibovitz's L-15 media and RPMI 1640 with 20% fetal calf serum with glutamine (2x) and penicillin/streptomycin (1x).
Further, broodstocks are often maintained in equal sex ratio (or may even be male-biased; e.g., Gold et al. 2008), which does not account for the limiting effect females of lottery polygynous species have on Nb.
Our study system was the white seabass (Atractoscion nobilis Ayres 1860) replenishment hatchery in Carlsbad, California, USA, where four breeding groups of 50 fish each are maintained in equal sex ratio (Bartley et al. 1995; see 'Supporting information' for a history and description of the program).
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MCF-7 cells were maintained in medium consisting of equal parts phenol red-containing DMEM and Ham's F12, 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA).
Worms from the resulting [ fog-2] introgression strains were then mixed in equal proportions and maintained in a large population to mate freely for at least six generations, in order to recombine the genomes and thereby create the HC-founding stock.
To create the NC-founding stock in parallel, self-fertile hermaphrodites and males from the original twelve geographic isolates were mixed in equal proportions and maintained in a large population to mate freely for at least six generations, in order to recombine the genomes and thereby create the NC founding stock.
They are based on the pCM62 and pCM66 plasmids that were shown to be maintained in M. extorquens AM1 with equal efficiency [25].
When VPA was given as maintenance therapy for childhood malignant glioma after postoperative combined chemotherapy and irradiation, about 10% of these patients were maintained in continuous complete remission and an equal number of patients showed at least partial responses (Blaheta et al, 2005).
An equal number of samples were maintained in osteogenic conditions but did not undergo Raman analysis.
As a negative control an equal number of wells were maintained in expansion media for 20 days.
The maximum amount of plutonium-239 that can be indefinitely maintained in an adult without significant injury is 0.008 microcurie (equal to 0.13 microgram [1 microgram = 10−6 gram]).
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