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Cells were maintained in enriched N2 medium with 10 ng/ml of basic fibroblast growth factor (R&D systems, Minneapolis, MN, USA) added every 24 h and medium changed every alternate day to keep the cells in an undifferentiated and proliferative state.

15, 16 We demonstrated that mice maintained in enriched environments generally performed well on the spatial memory tasks on the Morris water maze and perfectly distinguished between old and recent and between displaced and stationary objects in the episodic-like memory tests, whereas individual mice maintained in impoverished cages lost those abilities.

To this aim, BrdU injections were performed after 3 weeks of initial sensory enrichment and animals were maintained in enriched conditions for additional 10, 21 or 42 days (EN-1, EN-2 and EN-3a respectively; Fig. 1A).

A group of animals maintained in enriched conditions for 42 days before returning in standard housing for 21 more days (EN-3b; n = 7) was also performed.

An experimental group represented by animals maintained in enriched conditions for 21 days after BrdU injection and returned to standard housing for 21 more days (EN-3b) was added to the long-survival time point (Fig. 1A).

Bacterial strains (S. aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853) were maintained in enriched tryptone soy broth (TSB, BIOMED) and kept at 4 °C.

Similar(54)

The cells were maintained in N-2 medium enriched with 10 ng/ml basic fibroblast growth factor (bFGF; R&D systems, Minneapolis, MN, USA) added every 24 h.

The Krebs' solution enriched with oxygen was maintained in a 37°C water bath and was circulated through the center chamber at a flow rate of 2 mL/min.

We showed that in the absence of serum, addition of basic fibroblast growth factor maintained and enriched FGFR3-positive cells.

The Fig4 plt allele was maintained in a mixed strain background whereas the Fig4 Floxed/Floxed, P0-Cre genotype was enriched in the C57BL/6J strain (3, 12).

In roots, the most significantly enriched GO term was "localisation", which corresponds to any process in which a substance or a cellular entity is transported to and/or maintained in a specific location.

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