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Marine organisms can be maintained in either natural or artificial seawater; the latter has the advantage of being initially free from disease-causing organisms and pollutants but may not be as suitable for some organisms.
Compared with wild-type cells, the amount of Sir proteins is comparable in sir1Δ or spt16-m cells and the similar "M" pattern was observed, indicating that the silencer function is largely maintained in either single mutant strains.
Cells maintained in either media demonstrated comparable proliferation rates and differentiation capacity toward osteogenic and adipogenic lineages.
Cells were maintained in either serum-free (0%) myocyte media (80% DMEM, 20% M199) or myocyte media supplemented with 5% fetal calf serum (FCS).
Cells were maintained in either Dulbecco's modified Eagle's medium (DMEM) or Eagle's minimal essential medium (MEM) in the presence of 10% fetal bovine serum (FBS).
Cells were maintained in either normal culture media (DMEM supplemented with 10% FBS, 10 µg/ml insulin, and 100 U/m Pennicillin/Streptomycin) or L-Cell (Con) or Wnt-3a (Wnt) conditioned media [73] diluted 50% with normal culture media.
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Cells were maintained in medium (either MEM, or DMEM-F12) with 5 or 10% foetal bovine serum and L-glutamine, in a humidified incubator at 37°C with 5%-CO2.
Cell-based cartilage repair strategies include enhancement of endogenous repair mechanisms by recruitment of stem cells from the bone marrow following perforation of the subchondral bone plate; osteochondral implantation; implantation of chondrocytes that are maintained in defects by either a membrane cover or scaffold, and transplantation of mesenchymal stem cells into cartilage lesions.
However, cycles were not maintained in L/L at either temperature (Figure 9).
MMECs were maintained in media containing either NG or HG alone or with metformin and stained with DHE for the measurement of ROS.
Wild-type (WT NSCC were isolated from E14.5 embryos and maintained in culture either under proliferating conditions, or induced to differentiate.
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