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Zebrafish embryos were maintained in egg water at 28.5°C and were fed at 5days with NOVO TOM and with live arthemia at 11 days of life.

To prevent melanization, larvae were generally maintained in egg water supplemented with 0.003% PTU (1-phenyl-2-thiourea; Sigma-Aldrich).

Zebrafish embryos were maintained in egg water at 28.5°C and were fed at 5 days with NOVOTOM and with live artemia at 11 days of life.

Embryos were maintained in egg water (60 mg/L Red Sea salts, 2 mg/L methylene blue) until the appropriate stage.

For all experiments, zebrafish embryos [Tupfel long fin (TL) or tp53zdf1] were maintained in egg water consisting of 0.03% Instant Ocean (Aquarium Systems, Inc., Mentor, OH) in R.O. water at 28.5°C.

Similar(55)

Therefore, MAPK activity was maintained in activated eggs by MEK-EE.

Since RVF virus can be maintained in mosquito eggs for extended periods and transmitted under favorable conditions (6 ), the high magnitude of NDVI and rainfall patterns reported should prompt heightened veterinary and human surveillance for RVF in coastal Arabia and mass vaccination of susceptible animals.

Eggs maintained in aquariums hatch in approximately five and a half months, with the hatching shark measuring 14-15 cm long.

The relatively big, raptorial, marine batrachoid fish might intuitively not look like a very good laboratory model, but the hardy adults are readily maintained in many laboratories, and eggs and larvae are also easily accessible and available.

At these stages, spindle size is small compared to cell size, and cytoplasmic mechanisms likely operate, since in vivo spindle size is maintained in extracts prepared from eggs or two-cell stage embryos (Mitchison et al., 2005; Wuhr et al., 2008; Loughlin et al., 2011).

Late embryos and hatchlings (stages 29 and 30), respectively, were obtained from squid egg fingers maintained in tanks with fresh running seawater at 20°C and staged according to Arnold Arnoldd, 1965).

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