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Control roots were maintained in distilled water.
They were maintained in distilled water in petri dishes at room temperature.
All isolates were collected between 1973 and 2013, and maintained in distilled water.
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For each patient, fresh and vitrified/warmed samples were fixed in a 4% paraformaldehyde solution in distilled water maintained at pH 7.4 overnight at 4°C.
Then, the worms were washed in distilled H2O.
Pb acetate was made to 3 mM in distilled H2O.
Endogenous peroxidase activity was quenched with 3%H2O22 in distilled H2O.
Working solutions were prepared by appropriate dilution of the stock solution in distilled water.
All compounds used in this analysis were solubi-lized in distilled H2O/MeOH.
Inorganic phosphate release was quantitated using a standard curve generated with KH2PO4 in distilled H2O.
The reverse transcription reactions were then diluted 1 5 in distilled H2O.
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