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Chromatin was isolated from OPCs and from cells maintained in differentiation medium for 5 days.
The 3T3-L1 cells were maintained in differentiation media for 3 days.
Alginate suspension cultures were established [31] and maintained in differentiation medium (CDM, Lonza) and ascorbic acid (100 µg/ml).
Luminometric analyses of lysates from cells maintained in differentiation medium revealed a time-dependent increase in luciferase activity, which correlated with the time-dependent rise in the frequency and size of myotubes.
The mRNAs were diluted with Opti-MEM I medium (GIBCO) before using Lipofectamine 2000 (Invitrogen) to transfect them into myotubes that had been maintained in differentiation medium for 4 d; 1 µg mRNA was used per 3.5 cm dish of cells [48].
Control cells were maintained in differentiation medium.
Similar(47)
The cells were maintained in the differentiation medium throughout the entire time period of differentiation.
For osteoblast differentiation, BM-MSCs at 100% confluence were maintained in osteoblast differentiation medium (10 nM dexamethasone, 100 μM L-ascorbic acids, 10 mM β-glycerophosphate I, and 2% FBS in DMEM complete medium ) for 3 4 weeks.
These differentiated mES cells were maintained in the differentiation medium until they were harvested for nuclear injection.
Subsequently, the cells were maintained in the differentiation medium for 5 days until exhibiting a fully differentiated phenotype with massive accumulation of fat droplets.
In comparison, those maintained in adipogenic differentiation medium were observed to continue to proliferate only to monolayer confluence regardless of oxygen tension.
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