Sentence examples for maintained in defined from inspiring English sources

Hepatocytes and other cellular elements isolated by collagenase perfusion of the liver and maintained in defined culture conditions undergo a series of complex changes, including apoptosis and cell proliferation, to reconstruct tissue with specific architecture.

Cells were then maintained in defined medium in the presence of 20 ng/ml of FGF2 from day 8 [7].

We have previously shown that hESCs can be maintained in defined conditions for prolonged time periods while retaining their pluripotency, and our current experiments suggested that each of the remaining steps could utilize such a line to generate dopaminergic neurons.

Moreover, additional non-polar or hydrophobic residues are also maintained in defined positions.

The pluripotent cell lines were maintained in defined medium mTeSR1 (StemCells Technologies) on Petri dishes coated with matrix Matrigel (BD).

The human normal oral keratinocytes (HNOKs) cell line was cultured and maintained in defined keratinocyte-SFM (Gibco BRL, Gaithusberg, Germany).

Established liver epithelial cells (WB cells; Hep3B cells) were maintained in a defined, serum-free medium control (70 μM choline) or choline deficient medium (CD, 5 μM choline) and the localization of connexin 43 protein (Cx43) was studied by immunocytochemistry and Western blotting.

Wild-type (WT) C57BL/6J and FVB mice were bred and maintained in our defined flora facility.

Human telomerase-immortalized corneal epithelial (HUCL) cells [38], [39] were maintained in a defined keratinocyte serum-free medium (SFM; Invitrogen Life Technologies, Carlsbad, CA) in a humidified 5% CO2 incubator at 37°C.

Following enzymatic dissociation, keratinocytes were seeded onto collagen IV (10 mg/ml -coated culture plates and mg/ml -coated culturel defined medium MCDB 153 (Eplates, Candade Biologics, Inc., Portland, OR, USA), with maintainednge twine a week, as previously reported [48].

hESCs were also maintained in a defined medium as indicated.