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Ticks were reared and maintained in chambers with a relative humidity of 80% 90% at 22°C before feeding on artificial skin.
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Inoculated fruits were maintained in moist chambers (95 100 % relative humidity) at 28 ± 2 °C.
Coquillettidia cultures were maintained in dark chambers at 12±1°C and 70±2% RH.
Animals were maintained in climate chambers (Sanyo incubator MIR-253), in which air was filtered and light was provided by cool fluorescent lamps.
The plates were sealed with Parafilm and maintained in growth chambers at 25 °C.
Whiteflies were maintained in climate chambers at 27 ± 1 °C, a photoperiod of 14 h/10 h and 70 ± 10% relative humidity.
The pots were maintained in controlled chambers at 24±2°C, 60% relative humidity, with a 16-h light/8-h dark regimen.
A. gambiae G3 strain was reared and maintained in humidified chambers at 28°C with a 12 h light/dark cycle.
An. gambiae G3 strain was reared and maintained in humidified chambers at 28°C with a 12 h light/dark cycle.
After feeding and detachment, all ticks were maintained in humidity chambers to enable molting or laying of eggs.
The flow of sterile air was not subject to control and maintained in both chambers without interruption.
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