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The cell lysates were maintained in cell lysate collecting chambers through the through-hole at the bottom of each microwell.
The dental pulp and periodontal constructs (n = 144) were maintained in cell culture for between 3 and 14 days.
These gels were subjected to a cyclic strain of 10% superimposed on two separate tare loads of 2 and 10 mN, while being maintained in cell culture conditions.
Cells were grown and maintained in cell culture plates at 37°C in 95% air and 5% CO2 humidified incubator.
The IPEC-J2 cell line is a non-transformed intestinal porcine epithelial cell line continuously maintained in cell culture.
The cell lines TRAMP-C2, DU145, PC3, LNCaP and Hela were maintained in cell culture medium and split using trypsin digestion when 80% confluence was reached.
Similar(30)
At the end of the deposition procedure, the samples were rinsed in 1× PBS and subsequently maintained in cell-culture medium at 37°C.
However, heterochromatic localization is maintained in cells with severely decreased levels of DNA methylation.
This pattern of Rst2 localization was maintained in cells with low or high Tor2 activity, indicating that Tor2 does not regulate Rst2 subcellular localization.
Therefore the protein resistance of POEGMA-brushes is maintained in cell-culture conditions for long periods of incubation.
In general the balance between ROS generation and elimination is delicately maintained in cells.
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