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After emergence, adults were maintained in cages, in the same locale.
Adult mosquitoes were maintained in cages with 250 mosquitoes per cage of size 128 oz, with an equal sex ratio.
The animals were maintained in cages under biosafety-level-3 conditions at the Institute for Scientific Public Health (ISP), Brussels, Belgium.
Adult flies were maintained in cages at 28°C under a 12 h light∶12 h dark photoperiod on artificial diets consisting of 2.5% yeast extract, 7.5% sugar, 2.5% honey, 0.5% agar and 87% H2O.
Male wild-type C57Bl/6J mice (age: 6 weeks, body weight: between 20 and 25g) were maintained in cages with a 12h∶12h light-dark cycle starting at 6 00 in a room at 22°C.
Mice were maintained in cages fitted with microisolators connected to negative pressure.
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Flies have been maintained in cage culture at 25°C on a 12 12 light/dark cycle, at high population size (>200 individuals) to minimize inbreeding.
Since transportation back to the UK, flies have been maintained in cage culture at high density (>200 individuals) with an ∼1 1 sex ratio to minimise inbreeding.
Rats were maintained in individually ventilated cages (4 per cage) in the Animal Holding Facility (AHF) at the International Medical University (IMU) after their arrival.
All animals were maintained in individually ventilated cages.
Mice were maintained in individually ventilated cages (IVC) (Allentown Inc., NJ, United States).
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