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Flies were maintained in bottles on standard food at 25° on a 12 hr:12 hr LD cycle.
Small-scale cultures (25 mL) were maintained in tubes with an inner diameter of 2.2 cm and large-scale cultures (800 mL) were maintained in bottles with an inner diameter of 9.5 cm.
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Flies were maintained in bottle cultures on a rich dextrose medium at 25° and in 12 hr:12 hr light/dark for the duration of the experiment.
Cells were maintained in roller bottle cultures for at least two feedings prior to transfection by electroporation, as previously described [39].
For example, a stock could be a population maintained in a bottle in the lab composed of a mixture of multiple genotypes, or could be an inbred line of a plant whose genotype is fixed.
For starvation experiments, exponentially growing bacteria were washed, resuspended in PBS supplemented with 0.025% Tyloxapol, and maintained in roller bottle culture for a further 24 hr (Gengenbacher et al., 2010).
Fly stocks were maintained in 250 ml bottles in uncrowded conditions.
Batch cultures of K. brevis (Wilson isolate) were maintained in 1 L bottles in f/2 medium using 20 μm filtered, autoclaved natural seawater (36 ‰) with the following modifications: ferric sequestrene was substituted for EDTA · Na2 and FeCl3 ·6H2O and 0.01 μM (final concentration) selenous acid was added.
These cells were maintained in 75 cm culture bottles, pre-treated for cell culture.
Throughout the experiments, serum bottles were maintained in the dark at 20 °C on a rolling shaker.
Between sampling the serum bottles were maintained in the dark at 20°C on a rotating shaker.
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