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Cells were maintained in appropriate medium conditions until harvested.
Twelve hours post infection, virus was removed by washing, and cells were maintained in appropriate media.
Jurkat T cell line JJK, HeLa-T4 and HEK 293T cells were maintained in appropriate growth media.
For experiments evaluating the effects of demethylation, cell lines maintained in appropriate media were treated with 5-aza-2'-deoxycytodine (5-aza-dC) (Sigma-Aldrich, St Louis, MO, USA) or DMSO for three to five days.
All cell lines were maintained in appropriate media RPMI-16400 or Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.
Since good tissue viability is critical for quantitative assessment of bioelectric responses our recommendation is that specimens are maintained in appropriate medium on ice until used in functional measurements, which should be performed immediately [ 13].
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Legal names are maintained in the appropriate systems of record, such as SAP for employees, WOOFA for faculty, or Jenzabar for students.
All corrective actions will be documented in writing and maintained in the appropriate personnel record.
Cells were grown in Dulbecco's modified Eagle's medium (DMEM) and Minimal essential medium with Earle's salt (EMEM), respectively, and supplemented with 10% fetal calf serum (FCS) and maintained in the appropriate medium with 1% FCS.
All stable cell lines were maintained in the appropriate selective antibiotics.
All cell lines were grown and maintained in their appropriate medium.
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