Sentence examples for maintained in a regular from inspiring English sources

The androgen-dependent LNCaP cell line was obtained from American Type Culture Collection (Rockville, MD) and was routinely maintained in a regular medium: phenol red-positive F-12 medium (Gibco) with 10% FBS (Biowest) at 37°C in 5% CO2.

The internodes were maintained in a regular refrigerator at a ~4°C during few minutes (0 h treatment), 24 and 60 h after field harvested.

In all these studies, the trees were planted into sites free of competing vegetation and maintained in a weed-free state through regular vegetation control until the trees were well established (12 18 months in eucalypts and 3 5 years in pines).

Red rice biotypes were collected on a farm in Cerro Largo, Uruguay (31°46′S, 54°26′W), and maintained in a greenhouse (approximately 25°C) with regular irrigation.

Upon collection, samples were placed on dry ice and maintained in a -80°C freezer until thawed on regular ice prior to chemiluminescent analysis.

In order to visualize astrocytes for patch-clamp recording, the slices were first incubated in warm ACSF (30 °C) containing 1 μM sulphorhodamin-101 (SR101) for 20 min and then transferred and maintained in the regular ACSF at room temperature until placed in the recording chamber.

Cells maintained in the regular culture medium served as controls.

Negative Control: Cells maintained in the regular cell culture medium for the entire experiment (DMEM with 20 % FBS, 1 % Pen-Strept, 1 % non-essential amino acids).

These cells were maintained in either regular replete medium or Earle's balanced salt solution (EBSS) as an autophagic stimulus and were then embedded in paraffin wax and processed as would be the case for ex vivo material.

LNCaP C-33/C-81 C-33/C-81 C-33/C-81lls were routinely maintained in the regular medium, i.e., phenol red-positive RPMI 1640 medium supplemented with 5% FBS, 2 mM glutandne anDU50 µg/ml gentamicin.

In addition, consistent with the previous findings (Fig. 4B), parallel co-cultures of myoblastsFLPe and myoblastsGS.Luc maintained in regular growth medium did not show a time-dependent rise in luciferase expression despite the increase in cell numbers due to mitosis (Fig. 5B, solid bars in right panels).