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A two-stage culture was maintained in a constant temperature shaker as follows: A, cultured at 30 °C for the first 6 days and 35 °C for the last 2 days; B, cultured at 30 °C for the first 4 days and 35 °C for the last 4 days; C, cultured at 35 °C for the first 4 days and 30 °C for the last 4 days; D, cultured at 35 °C for the first 6 days and 30 °C for the last 2 days.
The coil was maintained in a constant position with the Medronic coil arm.
Animals were maintained in a constant environment and had unrestricted access to food and water.
All crickets were maintained in a constant temperature room (25°C), maintained on a 12 12 h light:dark cycle.
One-year old C57Bl/6J mice were maintained in a constant light/dark cycle (12 h/12 h).
These strains have been maintained in a constant laboratory environment at 20°C on standard dextrose/yeast/agar medium.
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Because D. melanica can be maintained in a state of constant clonal reproduction in the lab, it is straightforward to utilize standard quantitative genetic techniques to estimate the contribution of genetic and plastic phenotypic effects underlying adaptive traits.
The rats were maintained in a quiet room at constant temperature 20-22°C 20-22°Cumidity (50-60%) with 12 h light–dand cycle.
The plates were then maintained in an incubator at a constant temperature of 44 °C for 24 h.
C. intestinalis was collected in Aburatsubo Bay (Kanagawa, Japan) and maintained in an aquarium under a constant light condition until use to prevent spontaneous spawning.
Treated and control individuals of both species were maintained in a temperature-controlled chamber at a constant 20 ± 1.5 °C after treatment.
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