Sentence examples for maintained frozen until from inspiring English sources
Samples were stored at –70° C; shipped frozen overnight to Athens, Georgia; and maintained frozen until analyzed.
The strain was last stored in 1988 and was maintained frozen until shipping to Oxford in 2008.
Culture medium was centrifuged at 4°C for 10 min at 10,000 × g, divided into aliquots, immediately frozen at −80°C, and maintained frozen until analysis for cytokines and β-human chorionic gonadotropin (β-hCG).
Sections were placed on 25 mm×75 microscopecroSuperfrostrfrost Plus slides (Fisher), and maintained frozen at −80°C until staining.
Fetuses were removed from the uterus, and whole brain tissues were removed, individually snap frozen in liquid nitrogen, and maintained frozen (< −68°C) until shipment for analysis.
Two 1 mL aliquots of the supernatants were transferred to screw-cap storage tubes and maintained frozen at −80°C until assayed.
Overnight-fasting serum and plasma samples (from whole blood preserved using EDTA and NaF) were maintained frozen at −20°C until analysis.
The isolates were identified by conventional biochemical tests (coagulase) and commercial identification galleries (API-STAPH; bioMérieux, Marcy L'Etoile, France) and were maintained frozen at –20ºC in skimmed milk until experiments were performed.
The isolates were maintained frozen at -20°C in Trypticase soy broth (Difco Laboratories, Michigan, USA) containing 10% glycerol until testing.
Aliquots were maintained frozen at –70 °C.
The tumor can be re-grafted and maintained in vivo for many months; otherwise fragments were frozen until re-utilization.
