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The material was preserved in sterile plastic bags and maintained frozen at −20 °C.
Stock cultures in 25% glycerol were maintained frozen at −80°C.
The strain was maintained frozen at −80 °C with 20% (w/w) glycerol added.
All C. acetobutylicum and E. coli strains were maintained frozen in 15% glycerol at −80°C.
This valuable material can be maintained frozen for years and further used to perform independent experiments.
Collectively, these data indicate that viable BM cells can be maintained frozen, and once thawed, they can readily be used to generate BMDM.
Similar(37)
The results show that a significant reduction of up to 40% in the energy consumption per year can be achieved by implementing such concept whilst maintaining frozen body at desired level.
Participants transported samples to the CHBR weekly in coolers with cold packs to maintain frozen samples.
When performing diagnostic or epidemiological surveys, particularly in remote areas in resource-poor settings, the facilities for processing blood and maintaining frozen samples frequently do not exist.
The chancellor avoided raising fuel duty and maintained freezes on alcohol.
70 We have found that return of specimens using surface mail did not result in reduced sensitivity when compared to immediate and maintained freezing of specimens, even over long distances.
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