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The colony was maintained free of known and suspected murine pathogens.
All cells were cultured at 37°C in a humidified incubator containing 10% CO2 and were maintained free of mycoplasma.
Initially, plants were maintained free of endophytes by planting seeds or cuttings in sterile growth chambers (2 weeks).
All cells were cultured at 37°C in a humidified incubator containing 10% CO2 and were maintained free of mycoplasm.
Male Syrian golden hamsters (Mesocricetus auratus) 4 to 6 weeks of age were maintained free of pathogens with water and food ad libitum.
The cells were cultured and treated at 37 °C in a humidified incubator containing 5% CO2 and maintained free of mycoplasma as determined by Hoechst staining.
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Aside from supporting air and ground operations, the US Navy's focus has been on both maintaining free movement of shipping — i.e., oil tankers — in and out of the Gulf in general, and deterring Iran in particular from getting any bright ideas about messing with the free flow of the world's oil supply.
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In August 1985, Iowa joined 160 other ships for Exercise Ocean Safari, a NATO naval exercise aimed at testing NATO's ability to control sea lanes and maintain free passage of shipping.
Increasing amounts of Chinese commercial shipping to and from Africa will mean that China is going to be more heavily invested in maintaining free movement of its own shipping across the Indian Ocean, likely drawing the Chinese Navy further toward the Tanzanian coast whether they're planning for it right now or not.
Our main priority has to be to protect opportunities for Scottish students to study at Scottish institutions by maintaining free education north of the border".
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