Sentence examples for maintained for two passages from inspiring English sources

GG7 cells were pelleted by centrifugation at 1200 r.p.m. for 10 min and resuspended in Dulbecco's modified Eagle's medium high glucose (DMEM-Hg) (Gibco Life Technologies) medium supplemented with 10% FCS and 2% penicillin pen)/streptomycin strep) (Gibco Life Technologies) and maintained for two passages after which the concentration of pen/strep was reduced to 1%.

Dissociated cells were grown in culture with selective Quantum286 synthetic medium complemented with KGF and HGF (referred to "complete Q286 medium") on fibronectin-coated plates and maintained for two to three passages as previously described [ 22].

TF1 cells for transduction were thawed and maintained for minimum two passages before being transduced with lentivirus prrl-shSCR or prrl-shRPL5A or prrl-shRPL11A (Moniz et al., 2012) with an MOI of 10.

Cell lines were maintained in this growth medium for two passages, after which they were maintained in MCDB media.

Cages were maintained for five days.

Cells were expanded for two passages and then cryopreserved.

The passage portion consists of a couple of questions for passage one, a couple of questions of passage 2, and compare/contrast questions for two passages.

Each well was maintained as a separate clonally expanded line for four passages prior to analysis.

In this study, we demonstrate that fibroblasts also maintain an epigenetic positional memory even after culture expansion for three passages.

In this study, we demonstrate that although the distinct biochemical and histopathological features of H-BSE can be maintained for at least three passages in C57Bl/6 wild-type mice, the emergence of classical BSE properties may occur during serial passages in some of the animals.

After culture on primary ovine fetal turbinate (OFTu) cells for ten passages, the virus was named ORFV AH-F10 and maintained in liquid nitrogen for preservation.