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After 48 h of insect release, these trays were transferred to a high humidity chamber (> 90% RH) where they were maintained for two days for egg incubation and maggot establishment.
Flies were maintained for two days either on a 1S1Y diet or a 10S10Y diet to produce anoxia tolerant and anoxia sensitive flies.
Cultures were maintained for two days at 37°C in a 5% CO2 atmosphere and the medium was replaced every 24 h, starting 3 h after plating.
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General Kuzmuk, one of Mr. Kuchma's close allies, had maintained for five days after the tragedy that his military could not have caused the crash.
Cultures were maintained for seven days immersed in a medium composed of MEM (Invitrogen, Carlsbad, CA) supplemented with 10% FBS (Sigma, St Louis, MO), Hydrocortisone (0.4 mg/mL) (Sigma, St Louis, MO), and Cholera Toxin (0.1 nmol/L) (Biomol Int., Plymouth, PA) 50 µg per mL ascorbic acid (50 µg/mL) (Sigma, St Louis, MO), insulin (0.5 µg/mL) (Sigma, St Louis, MO).
Cages were maintained for five days.
Because primary inflammatory mechanisms can last for about one week, the treatment was maintained for seven days.
This protocol was repeated with another generation of mosquitoes except that 50 infected mosquitoes were maintained for twelve days post-infection.
Human umbilical cord blood (hUCB) derived progenitor cells were cultured on hOSTs at day 6 of differentiation, with co-cultures maintained for twelve days in the presence of growth factors specific for progenitor differentiation towards the megakaryocytic lineage.
To assay their differentiation capacity, sorted GFP CPCs were re-aggregated for 24 hours in suspension and re-plated on fibronectin coated tissue culture dishes and further maintained for seven days in ESC differentiation medium.
Intravenous ceftriaxone was maintained for nine days.
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