Sentence examples for maintained for three days from inspiring English sources

Eggs were then maintained for three days in 17°C oxygenated sea water prior to embryo fixation and dissection.

Seedlings were maintained for three days at 4°C and than grown under 16/8 hours light/dark cycles at 23°C in growth chambers.

After drug selection, a stable pool, called the Unsorted pool, was maintained for three days at a concentration of 1 × 10 cells/mL.

Owing to the negative frequency-dependent fitness and low frequency of BW4005 in the initial chemostat evolution experiment [ 19], we mixed BW4005.C6 with MC4100 at a ratio of 1 99 and this co-culture was maintained for three days.

For experiments in hormone-deprived conditions cells were maintained for three days in phenol red-free DMEM plus 2.5% dextran-charcoal-stripped foetal calf serum (Life Technologies, Barcelona, Spain) and then they were treated in the presence or absence of 4OHT.

General Kuzmuk, one of Mr. Kuchma's close allies, had maintained for five days after the tragedy that his military could not have caused the crash.

After 48 h of insect release, these trays were transferred to a high humidity chamber (> 90% RH) where they were maintained for two days for egg incubation and maggot establishment.

Cultures were maintained for seven days immersed in a medium composed of MEM (Invitrogen, Carlsbad, CA) supplemented with 10% FBS (Sigma, St Louis, MO), Hydrocortisone (0.4 mg/mL) (Sigma, St Louis, MO), and Cholera Toxin (0.1 nmol/L) (Biomol Int., Plymouth, PA) 50 µg per mL ascorbic acid (50 µg/mL) (Sigma, St Louis, MO), insulin (0.5 µg/mL) (Sigma, St Louis, MO).

Cages were maintained for five days.

Because primary inflammatory mechanisms can last for about one week, the treatment was maintained for seven days.

This protocol was repeated with another generation of mosquitoes except that 50 infected mosquitoes were maintained for twelve days post-infection.