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The transfection mixtures were removed at 6 h post transfection, and transfected cells were maintained for further processing.
Once features were obtained, we used a ranking algorithm ListNet (Cao et al., 2007) to rank gene IDs for each mention and the top one ID was maintained for further processing.
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The upper aqueous phase was transferred to a 2.0 ml tube, disinfected with Vesphene-II and taken out from BSL-3 for further processing and maintained on ice until RNA was precipitated on the same day.
The SEC sequences have then their signal peptide intervals removed from the original sequence, maintaining only the predicted mature protein sequences for further processing.
PSL is the storage period from the time of freezing for as long as the product maintains its organoleptic and nutritive characteristics and is suitable for human consumption or for further processing.
Contigs longer than 1,000 bp were used for further processing.
Images were imported into ImageJ for further processing.
Cell pellets were frozen at −80 °C until ready for further processing.
After each exposure, centrifuge tubes were kept on ice until ready for further processing.
These manually assigned labels were transferred back to the single cell level for further processing.
They were distributed for further processing and repackaging and will not have the company's establishment number on the package.
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