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In the macroencapsulation approach the animals showed euglycemia within 25 days, which was maintained for further 20 days, but after that the animals died.
The substrates were placed in the plastic chambers with high humidity maintained for further basidiomata production.
The transfection mixtures were removed at 6 h post transfection, and transfected cells were maintained for further processing.
An aliquot of 10ug of DNA was used for the sequencing via pyrosequencing (see below), and the remaining stock was maintained for further sequencing and completion of the gaps.
In addition, we have shown that efficient GDP detection is not dependent on a narrow region of finely tuned parameters, but rather, can be obtained for a wide range of different parameter values, provided specific parameter relationships are maintained (for further discussion of the parameter values, see Supporting Information S1).
Germinated seedlings were transferred to greenhouse and maintained for further analysis.
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Eventually, the iron modified carbons were heated at 105 °C, named Fe2+-PPC and Fe3+-PPC and maintain for further uses.
Upon reaching T G, the same flow of 100 sccm O2 was maintained for a further 1 to 4 h.
Upon reaching TG = 500°C to 700°C, the same flow of NH3 and H2 was maintained for a further 60 min; after which, the reactor was allowed to cool down slowly for at least 30 min without changing the gas flows.
The initial column temperature was held at 285 °C for 30 min and then increased at a rate of 10 °C/min to 300 °C and maintained for a further 5 min with a flow of 1.0 ml/min and 2 μl sample.
Upon reaching TG, the same flow of Ar was maintained for a further 60 min, after which the reactor was allowed to cool down slowly for at least 30 min without changing the flow of Ar.
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