After 4 days of RNAi treatment at 15°C, adult worms were either maintained at the permissive temperature (15°C) or shifted to the restrictive temperature (25°C), then examined 24h later for the suppression of the Clr phenotype.
For genetic screen using the Exelixis Df lines: approximately 5∼10 female virgins from either w ; GMR-Gal4, UAS-dE2f1RNAi #10 (or #8)/ CyO; +/+, or w ; ptc-Gal4, UASdE2f1dsRNA#3/CyO; +/+ lines were crossed with 5∼10 males from each Df line on second or third chromosomes, and the crosses were maintained at either 25° (for the eye phenotype) or 22∼23° (for the wing phenotype).
The pH was maintained at either 5.0 or 5.5 throughout the fermentations by automatic addition of 4 M NaOH.
The osteotomy is then completed with slope either maintained or flattened at the discretion of the surgeon.
Ed Bastian, Delta's president, told reporters that 80% of corporate clients surveyed by the airline "indicated their second half of the year spend on Delta will either be maintained at the same pace or increase on a year-over-year basis".
ULTI cultures were either maintained at 33°C in the presence of IFN-γ, or 37°C in the absence of IFN-γ for twenty-four hours, treated with either pifithrin-α 20 µM or DMSO vehicle for six hours, then (without washing) treated with either etoposide 25 µM or DMSO vehicle for twelve hours.
In all, 10 tumours (A2, A5, A11, A18, A19, A22, A23, B13, B26 and B27) had lost heterozygosity at either marker but maintained allelic balance at the other marker on 19q.
Johnson maintained the lead at the restart.
Either cancer cells preferentially maintain aneuploidy at the expense of increased cellular stress and slower proliferation rate or they might instead have also evolved to overcome cellular stress in response to aneuploidy.
They are maintained at the same extent.
