Sentence examples for maintained during culturing from inspiring English sources

The selection appeared highly stringent, and the plasmid was stably maintained during culturing.

The plasmid co-expressing rdpe and dal was introduced into dal mutant, selection appeared highly stringent, and plasmids were stably maintained during culturing.

However, a thorough characterization of the isolated hepatocytes is essential to ensure that functions of interest are maintained during culture.

Stable transfectants were selected by culturing cells in the presence of 200 μg/ml G418 (drug levels maintained during culture of cell stocks but removed for individual experiments).

To ensure elimination of differentiated cells, blasticidin selection for cells retaining Rex1 expression was initiated 48 hr after exposure to N2B27 medium and maintained during culture in 2i/LIF.

Importantly, upon transplantation, the ability of mammosphere cells to repopulate a mammary fat pad was maintained during culture at a frequency of 1 in 298 mammosphere cells (Additional file 1: Figure S1B and C).

This localization was also maintained during subsequent culturing (not shown).

Aliquots from this broth were used to inoculate wells in plates used for growth curve experiments in the Bioscreen apparatus as described previously, to ascertain if the inhibitor-resistant phenotype was being maintained during isolation and culturing.

Plasmid pUCPMS was maintained during batch culture and biofilm experiments by the addition of 50 µg ml−1 gentamicin; pMDGFP was maintained in E. coli and P. aeruginosa cultures by the addition of 50 µg ml−1 ampicillin and 400 µg ml−1 carbenicillin, respectively.

Treatment was initiated prior to vector exposure and maintained during subsequent co-culture with 293T cells.

There has been controversy whether regional differences amongst stem and progenitor cells are cell intrinsic and whether these differences are maintained during expansion in culture.