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The pH value was maintained during cultivation at 7.0 ± 0.1 by 2M NaOH, temperature and stirrer speed were kept constant at 37°C and 600 rpm, respectively.
Similar(59)
Without solvents, the three mutants Δ acrR, Δ fabR, and Δ marR were maintained during successive cultivation, but the mutant Δ fadR was gradually reduced and nearly disappeared in the ninth cultivation (Fig. 2a).
Based on the obtained results, one can hypothesise that PMA1 expression levels may not be critical for the adaptation towards acetic acid since the transient overexpression after 2 h was not maintained during the whole cultivation period.
Acutodesmus dimorphus was genetically engineered by the addition of two genes, one for enhanced fatty acid biosynthesis, and one for recombinant green fluorescence protein (GFP) expression; both the genes and their associated phenotypes were maintained during fifty days of outdoor cultivation.
Uptake and production of most metabolites was balanced, i.e. they were proportional to growth for the main carbon sources and produced metabolites and for other amino acids (Additional file 2).This means that metabolic steady state was maintained during the first 72 h of cultivation.
A second laboratory trial was carried out with the nine Chlorella strains to compare growth in freshwater- and seawater-based media and test their ability to grow when the culture temperature was maintained, during the light hours, at 40°C, a value easily reached during outdoor cultivation in closed systems when cooling is not applied.
This temperature was maintained during 5 h.
The stirring was maintained during the electrodeposition.
Sterility was maintained during all surgical procedures.
CPR maintained during this.
Yeast forms were maintained by cultivation at 37°C in Ham's F-12 medium as previously described [29].
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