PmO2 was maintained during desflurane-induced hypotension.
Sterility was maintained during all surgical procedures.
CPR maintained during this.
Antibacterial properties of the AM were maintained after cryopreservation and freeze-drying.
Frozen stocks of viable bacteria were maintained in cryopreservation fluid (Protect™; TSC, Heywood, UK) at −80°C.
It was maintained under cryopreservation and reseeds three times per week in our laboratory in PEPHS medium, supplemented with 10% (v/v) bovine serum and Diamond's vitamins-Tween 80 mixture [ 35].
To implement this strategy, the germlines of these founders need to be maintained through sperm cryopreservation.
For this purpose, current controlled rate freezers (CRFs) are suitable for controlling temperature, maintaining the rate of temperature during cryopreservation.
Only a small mount of liquid nitrogen is needed to maintain the metal plate at this temperature during cryopreservation.
A second normalization determined average production based on number of cells cryopreserved – therefore even cells that were destroyed during cryopreservation were accounted for here.
