Sentence examples for maintained differentiated from inspiring English sources
Pre-coating of Ln enhanced cell differentiation, as the maximum activity of a cell differentiation marker (alkaline phosphatase) was 2 3 times higher than on Fn-coated collagen, and maintained differentiated phenotype in long term (up to 21 days) culture.
After 9 months and 16 passages this diploid cell line maintained differentiated characteristics and a high proliferative capacity.
Finally the use of surface modifications to maintain differentiated cells in their terminal phenotype is covered.
Overall, we demonstrate heparin gel sandwich to be an excellent system for maintaining differentiated, polarized primary hepatocytes for over three weeks.
In conclusion, the findings described in this study make it possible to maintain differentiated primary mouse hepatocytes that also show GJIC in long-term culture for 30 days.
The sandwich culture of hepatocytes, between double layers of extra-cellular matrix (ECM), is a well-established in vitro model for re-establishing hepatic polarity and maintaining differentiated functions.
Typically, this is achieved through the use of degradable biomaterials to either induce surrounding tissue and cell ingrowth or to serve as temporary scaffolds for transplanted cells to attach, grow, and maintain differentiated functions.
In the current report, we identify an efficient means by which to maintain differentiated tenocytes in vitro by employing the hanging drop technique in combination with defined growth media supplements.
Extracellular matrix (ECM) scaffolds prepared from different tissue sources or using different methods have been demonstrated to have distinctive effects upon cell adhesion patterns and the ability to support and maintain differentiated phenotypes.
Hepatocytes in CS cultures remain stable over extended periods of time, and maintain differentiated hepatic functions [3], [4].
Cells morphology: The main problem we encountered with endometrium in vitro histoculture was to maintain differentiated cells and avoid they become fibroblastic.
