Sentence examples for maintained cell suspension from inspiring English sources

SW maintained cell suspension and tissue cultures, subcultured and regenerated transgenic colonies and participated in screenings.

The cells were then incubated in a 37°C incubator horizontally for 50 min with occasional tapping to maintain cells in suspension.

These HNSCC oraspheres were developed by maintaining cells under suspension conditions on poly-HEMA coated plates (7.5 mg/mL in 95% ethanol, Sigma-Aldrich, St . Louis MO) for 6 days.

The bioreactor, the rotating wall vessel (RWV /rotating cell culture system (RCCS) from Synthecon (http://www.synthecon.com/), is a cylindrical vessel that maintains cells in suspension by slow rotation around its horizontal axis with a coaxial tubular silicon membrane for oxygenation.

The cell suspension has some salts to maintain cell viability, and a tetrazolium redox dye chemistry to measure cell respiration.

For comparison with coculture, control PBMC-only incubations were performed in microcentrifuge tubes that were rotated slowly to maintain cells in a suspension state and minimize contact with plastic surfaces.

The cell suspension was diluted 100 times with 9 g L−1 NaCl solution to maintain cell integrity and dyed with 0.3 g L−1 methylene blue.

Ethidium+ (25 μM) was added to a cell suspension maintained at 37°C with stirring in a time-zero module, and 1 mM ATP was added 40 s later.

Cells were maintained as a monocytic cell suspension in T-75 flasks containing RPMI 1640 medium supplemented with 10% fetal bovine serum and antibiotics at 37°C in 5% CO2, and cultures were split every 3 to 5 days.

The perspectives are promising, as disposable bioreactors were successfully used for maintaining isoflavone-producing soybean cell suspension cultures.

The cell suspension was maintained by subculturing the 7-day-old cells (in stationary phase) as described.