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For some years it was thought that MreB and its relatives polymerized to form a continuous helical filament just beneath the cytoplasmic membrane and that this cytoskeleton-like arrangement established and maintained cell diameter.
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Under the operating conditions employed, both SPLITT and centrifugation maintained cell viability above 98%, but resulted in significant sublethal damage, including echinocyte formation, decreased cell diameter, decreased mean corpuscular hemoglobin, and membrane hyperpolarization which was inhibited by EGTA.
To maintain cell conditions.
MreB Orientation Correlates with Cell Diameter in Escherichia coli.
In addition, cell diameter was measured using a Leica Light Microscope M125 (Leica Microsystems).
For example, cell diameter only varies by a few percent across a given population.
Other correlations between MreB and cell diameter are not found to be significant.
Cell diameter (m).
Cell diameter is expressed as percentage normalised to the diameter of mock-treated cells, which was set as 100%.
(B ) Vacuole diameter is proportional with the cell diameter.
The cell diameter varies from 25to4545 μm.
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