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The tumor cells were maintained by weekly intra-peritoneal transplantation of cells.
The halophilic bacterial consortium CY-1 was maintained by weekly transfers in SSDM with 10% salinity and phenanthrene (100 mg/l) for 3 months to become a stable microbial consortium.
This parasite strain is routinely maintained by weekly passages in CF1 mice.
Subsequently, the established CTL lines were maintained by weekly stimulation with IL-2-supplemented culture medium [52].
Promastigotes were maintained by weekly transfers in M199 medium, pH 7.0, supplemented with 10% fetal bovine serum.
The cultures could be maintained by weekly sub-culturing to provide actively proliferating mid-log phase cells for initiating fresh cell suspension cultures.
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Chinese hamster V79 and CHO cells were maintained by twice weekly sub-cultivation in minimal essential medium (MEM) containing 10% fetal bovine serum (FBS).
Inter-rater reliability was maintained by formal, weekly 'curation meetings' where difficult publications, or any publication a curation team member felt would be useful for discussion and comparison, were reviewed conjointly.
The Type I T. gondii parasite strain RH was maintained by bi-weekly passage on human foreskin fibroblast monolayers (American Type Tissue Collection) in DMEM supplemented with 1% Bovine Growth Serum (BGS), 100 U/ml penicillin and 0.1 mg/ml streptomycin.
Embryogenic line M95, provided by Dr. Christian Walter (Scion, Rotorua, New Zealand), was proliferated and maintained by bi-weekly subcultures of individual clumps onto EDM6 medium [ 112].
Strain was maintained by bi-weekly transfer to R2A agar slants (HiMedia, India) and stored at -20°C in R2A broth amended with glycerol (20% v/v).
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