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Mice were kept at or above 85% of their free-feeding weight, which was maintained by supplementary feeding in addition to the food reinforcers in daily testing sessions.
A surgical level of anaesthesia was maintained by supplementary half doses of ketamine and xylazine injected every 45 min.
Animals were anesthetized by an intraperitoneal injection of 60 mg kg− 1 body wt sodium pentobarbital diluted in water (25% w/v) and maintained by supplementary injection of 10% of the original dose when necessary.
Animals were administered buprenorphine analgesic (0.03 mg/kg subcutaneously) prior to anaesthesia with sodium thiopentone (85 mg/kg ip), approved in Home office project number PPL 70/7055, protocol 4. Anaesthesia was monitored by reaction to foot pinching at 15 min intervals and respiration monitoring and maintained by supplementary infusions of sodium thiopentone.
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and xylazine (2 mg/kg, Rompun; Bayer Australia Ltd). and maintained initially by supplementary administration of ketamine and xylazine as required.
The only exception is the plastid class II aldolase (Patron et al. 2004), which is missing from the genome of C. velia (supplementary table S1 and fig. S3 e, Supplementary Material online) and of which the metabolic function in the Calvin cycle is probably maintained by a nonhomologous class I isoenzyme (supplementary fig. S3 d, Supplementary Material online).
Nucleotide conservation patterns in gltI and GcvB argue that this unusual RNA interaction has been maintained by selection and that the gltI-SroC-GcvB axis is functional in many enterobacterial species (Supplementary Fig S1).
Hart (1987) and Frankis (1988) also considered that their respective circumscribed Laricoideae is sister to Abietoideae rather than to the Pinus– Picea clade (fig. 1; supplementary table 1, Supplementary Material online) as posited by Price et al. (1987), whose view in turn was maintained by Farjon (1990), Wang et al. (2000), and Gernandt et al. (2008).
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