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After a series of gradual expansions, the cells were maintained by splitting every 4 6 days with change of media every 2 days.
Cells were maintained by splitting 1∶10 at 90% confluency.
Cells were maintained by splitting 1 10.
P19 mouse embryonic carcinoma cells (ATCC CRL-1825) (Glozandand Rogers, 1996) were cultured in minimal essential medium (MEM) with 10% fetal bovine serum and antibiotics, maintained by splitting 10-fold every 2 days.
All three S. rosetta cell lines (ATCC 50818, RCA, and Px1) were grown in cereal grass infused seawater at 25°C and maintained by splitting cultures 1 10 into fresh medium every 3 days (King et al. 2009).
Knockdown was maintained by splitting and re-transfecting cells with cavin-3 siRNA on days 5, 9, and 12. Cells were then serum starved for 20 hr and RNA from cells was either harvested immediately or following treatment with 100 ng/ml EGF for 1 or 3 hr.
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After widening into a divided highway maintained by the NJDOT, County Route 501 splits from the route by heading north on Kennedy Boulevard at an interchange.
It is maintained by caucus staff.
Maintained by us, for us.
Record maintained by: Dot TK Domain Registry.
It is maintained by Rich Meislin.
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