In intact cells, respiration is maintained by endogenous plasma-derived substrates only.
Because ATP production via aerobic respiration is maintained by a stoichiometric equation C6H12O6 + 6O2 → 6CO2 + 6H2O + 32ATP the rate of CO2 production is a proxy for the rate of ATP production (Lighton 2008).
When the animal started resisting the respirator, anaesthesia was maintained by phenemal (20 mg/mL) iv, the respirator disconnected, and the tracheal tube removed to obtain spontaneous respiration through the nose.
Animals were administered buprenorphine analgesic (0.03 mg/kg subcutaneously) prior to anaesthesia with sodium thiopentone (85 mg/kg ip), approved in Home office project number PPL 70/7055, protocol 4. Anaesthesia was monitored by reaction to foot pinching at 15 min intervals and respiration monitoring and maintained by supplementary infusions of sodium thiopentone.
After anesthetization by intraperitoneal injection of 1% pentobarbital sodium (Urchem, China), the respiration of the rat was maintained by a computer-controlled small animal ventilator connected to the rat through a tracheal cannula.
Together, these findings indicate that, under conditions of Nrf2 deficiency, ATP is produced primarily in glycolysis, not oxidative phosphorylation and the ΔΨm is largely maintained by the hydrolysis of ATP by the F1F0-ATPase, rather than by respiration.
Our hypotheses need no special mechanism to maintain respiration, because respiration is what is driving the symbiosis throughout.
Its energy charge is maintained by the metabolic network and restored primarily by glycolytic fermentation and respiration (Bolanos et al., 2010; Grüning et al., 2010).
Mitochondria were dysfunctional and fragmented, with impaired respiration, reduced respiratory chain complex activities, and a decreased potential maintained by reversal of the ATP synthase.
It is maintained by Rich Meislin.
