We obtained written consent from each study participant, and maintained confidentiality by analyzing the data in aggregate.
We determined whether primary human OA chondrocytes and chondrocytes passaged once used in these studies maintained their phenotype – by analyzing the expression of type-2 collagen, aggrecan, and SOX-9 mRNA, which are considered to be signatures of the chondrogenic phenotype [ 44].
We analyzed data maintained by the Food and Water Microbiology Laboratory, SGH, because it was the only public sector laboratory with reliable records.
To learn about the cause and detailed content of this polymorphic region, sequences of the IGHV region from the three human genomic sequence assemblies http://www.ncbi.nlm.nih.gov/genome/guide/human/release_notes.html in the database maintained by the National Center for Biotechnological Information (NCBI) were analyzed.
To study maintenance of the tracheal epithelium, we first tested whether homeostasis was maintained during our time course by analyzing cell proliferation, composition, density, and tracheal size.
Gene analyses and the working model for scytonemin biosynthesis was developed by TS, Chlorogloeopsis sequences and UV experiments were done by KP, cultures were provided from and maintained by RMP, and the pathway was analyzed by QG.
Cell viability in extended culture was maintained as analyzed by expression of Cell Tracker green and negligible ethidium homodimer-1 (a marker of cell necrosis) staining.
Spatially referenced data on individual residence and homestead exposure features were maintained and analyzed by using ArcGIS version 9.2 (ESRI, Redlands, CA, USA).
We analyzed clinical and epidemiologic data maintained by the Communicable Diseases Division, MOH, during 2000 2009.
We analyzed information provided in the National Electronic Injury Surveillance System (NEISS), maintained by the US Consumer Product Safety Commission (CPSC).
