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Each compound maintained binding affinity for NTR1, however, only KH24, KH26, and KH28 (as well as KK13) elicited significant hypothermic responses after oral administration.
A similar experiment with the P−Z+ peptide that maintained binding to the PDZ1 domain produced a peak in the elution profile at the expected elution volume of DegP12 cages (Fig. 4B).
Unaltered binding affinity to the original antigen was demonstrated by ELISA and maintained binding specificity was proved by Western blotting and immunohistochemistry. Since monoclonal antibodies against prion protein can antagonize prion propagation, humanized scFv specific for the pathogenic form of the prion protein might become a potential therapeutic reagent.
Interestingly, all mutants maintained binding to the N-terminal PLK4 fragment spanning residues 1 570.
Surface plasmon resonance analyses showed that E275C gp120 maintained binding affinity for sCD4 and 17b, the two canonical gp120 ligands.
R11-scFv-Fc-Sec conjugation was well tolerated as conjugates maintained binding to ROR1 expressed on HBL-2 cells.
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From this library, we isolated many variants that can bind a secondary antigen while maintaining binding towards their primary antigen HER2.
There are many advantages of using these small, stable domains that maintain binding capabilities similar to that of antibodies.
Among them, tartaric acid derivative (5) exhibited the most effective reduction of nonspecific binding proteins, whilst maintaining binding of the target protein.
After receiving the PBU message, the LMA updates the routing states and maintains binding caches and then replies with a proxy binding acknowledgement (PBA) message to the MAG. 7.
It is shown that the insertion of a ΨMeMepro at position 5 (Thr5CsA) maintains binding to cyclophilin A as well as to calcineurin and shows a 5-6 cis amide bond with all remaining amide bonds trans.
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