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We have successfully maintained beating hearts for up to 4 days without any contamination.
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The possibility of maintaining beating hearts ex vivo allows recording of acute and chronic effects on the cardiac function and viability.
The simple protocol for ex vivo culture allowed maintaining the beating heart for several days.
Although the differentiation of stem cells into cells of cardiac lineages in these hydrogels would be more indicative of their application in myocardial regeneration, it is of note that hydrogel was able to maintain the beating ability of differentiated cardiomyocytes in vitro.
In head racing, the rowers start 10 seconds apart, so Tucker had that margin to maintain to beat all comers.
Count your reps for round 1 and see if you can maintain or beat that number through rounds 2 through 4. To see the Prisoner Squat: https://youtu.be/P0rGQzZRnsM.
"Vulture Piano," from the new album, was the rare Xiu Xiu song that maintained its beat all the way through, even if the beat was sometimes buried in percussive metallic clangor.
Directors such as Gus van Sant and Steven Soderbergh dart between commercial and personal film-making; Jarmusch has maintained his beat-cool and found a younger generation of filmgoers.
Furthermore, this hydrogel was able to maintain the beating capability of the cells (see supporting data (video) in supplementary material available at doi 10.1155/2012/285678).
LeBron James scored 40 points and took over in the fourth quarter as the Cleveland Cavaliers, fueled by a home crowd that booed every move by Noah, maintained homecourt advantage by beating the Chicago Bulls, 112-102, on Monday night to take a 2-0 lead in the Eastern Conference playoffs.
A strong emphasis on rhythm is therefore common to most styles of EDM, while ambient music, which is less focused on maintaining a beat, provides an aural cushion for settling down at the end of the night.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com