Using genetically encoded indicators including GCaMP5 and GCaMP6s delivered by AAV2/1 into the visual cortex, we demonstrate that high-quality two-photon imaging of large neuronal populations can be achieved and maintained in awake monkeys for months.
Previous studies have demonstrated that cortex can maintain an awake state even when thalamus is lesioned or inactivated (Constantinople and Bruno, 2011; Zagha et al., 2013); our results therefore suggest that slow waves in the intact brain require the involvement of cortical, TRN, and TC neurons in a coordinated rhythm.
During the day, however, light levels should be maintained and patients kept awake if possible as one strategy to consolidate sleep at night.
According to the results in the literature about energy consumption in sensor networks [18], the main source of energy spent in a sensor is the power dedicated to maintain the sensor awake.
He also wondered "what Awake would be like in a Michael Britten-like alternate reality where the show lasted five seasons, or 10, or even just one more year", Could Awake maintain this story for long and still be compelling?" Bonaime wrote that it was "better to have one great season and be remembered fondly".
Of note, there is an increased tendency to maintain those patients awake, and spontaneously breathing, without the need for intubation [ 54].
Rats were maintained under a heat lamp until awake.
All patients, maintained in spontaneous breathing all through the procedure, received awake intubation performed by one of three anesthetists expert in difficult airway management.
Therefore, in the Japanese guidelines for awake craniotomy, the anticonvulsant level preceding surgery should be maintained within a therapeutic range [8].
Experienced teachers may simply argue that it is easier to keep students awake by maintaining the dialogue rather than finishing a PowerPoint monologue.
