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The viral genome is maintained autonomously in proliferating cells and each viral episome is replicated once per cell cycle during S phase [1], [2].
In DT40 cells, the tet-O HAC was maintained autonomously without integration into chromosomes (Fig. 2C).
These data show that STAR elements have spread through and been maintained autonomously within each staphylococcal genome.
FISH analysis showed that the HAC was maintained autonomously without any detectable integration into the host genome in three out of five randomly selected clones.
FISH analysis showed that the HAC was maintained autonomously in six out of six randomly selected clones (Fig. 4B and Supplementary Table S1).
The data show that STAR elements are associated with distinct flanking genes in each staphylococcal species, suggesting that they are maintained autonomously within each species, and that their positions within each genome are stable over time.
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Until yesterday DeepMind had maintained it operates autonomously from Google, with founder Mustafa Suleyman writing in 2016 that: "We've been clear from the outset that at no stage will patient data ever be linked or associated with Google accounts, products or services".
In HCT116 cells, the mini-EBV genomes wt-oriPp2908 and ΔDS p2910) are autonomously maintained, whereas extrachromosomal copies are not established in the Orc2-hypomorphic HCT116 cell line e83 (Fig. 4A).
Multiple stem cell populations residing in autonomously maintained compartments facilitate this task.
Successful transformation of this construct into B. burgdorferi results in P66 expression from the autonomously maintained shuttle vector.
Consistent with our results, they further provided evidence that, once set by the action of Piwi proteins on chromatin, a transposon silencing cannot be autonomously maintained.
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