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N-acetyl glucosamine (NAG) was added at a concentration of 50 mM to kill asexual stages at approximately day 8 [32]–[34] and maintained at this concentration throughout the period of gametocyte culture.
The concentration of applied NaCl was increased by 3 dS m-1 at each watering time to avoid abrupt osmotic shock, up to a final rate of 18 dS m-1, and maintained at this concentration until assessment.
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Cells were maintained at this drug concentration until their growth rate approached that of untreated OCI-AML3 cells.
Each bacterial culture was maintained at the concentration of 10 CFU/ml.
This difference is maintained at each concentration of u-PA in the lower well of the migration chamber.
The influent COD concentration was maintained at an average concentration of 6,240 mgCOD/L from the beginning.
Cells were maintained at a concentration of about 7 10×105 cells ml−1 throughout the experiment.
For bulk selection of stably transfected cells, cultures were expanded in the presence of 1 mg/mL G418 and maintained at a concentration of 0.2 mg/mL G418.
The cultures had been reared in the laboratory following the methods described by [25] and were maintained at a concentration of 1,000 cells ml−1 in each aquarium.
Cells were maintained at a concentration 1 × 10 cells/ ml, and were passed every three days.
The kinetic parameters for NaF were determined with SAM maintained at a concentration of 0.4 mM and NaF at increasing concentrations from 0 to 20 mM.
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