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The protein contents of WD-3 and Hopum, which were 7.41% and 6.44% before treatment, respectively, significantly decreased to 5.25% and 4.82% 40 days after treatment, and then maintained at this amount until 90 days after treatment without significant change.
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Unfortunately, things aren't maintained at this intense pitch.
Salinity was maintained at this level thereafter.
The amount of cytoplasmic β-catenin is maintained at a low amount via ubiquitin-proteasome-mediated phosphorylation and is regulated by a destruction complex contained of AXIN1, adenomatous polyposis coli (APC), casein kinase 1 (CK1), and glycogen synthase kinase 3β (GSK3β) [ 5].
This analysis is based on the Lande (1983) model that consists of a locus of major effect on the trait and treats the remaining loci of minor effects as genetic background (such that background variation is maintained at a constant amount).
Expression vectors were maintained at constant total amounts via the addition of empty vectors.
In order to ensure minimal decrease in the volume of the culture broth owing to sampling from the same flask, the total sampling volume was maintained at <10% of the total amount of the initial culture medium.
The C/N ratio in each flask was maintained at 10.0 with appropriate amounts of glucose (carbon substrate) and ammonium chloride (nitrogen substrate).
The carbonization temperature of NMCs was maintained at 800 °C so that the amount and type of nitrogen functionalities were constant.
Here, a homogeneous solution is prepared from 5% sodium chloride (purity > 99%, Merck KGaA, Darmstadt, Germany) and 0.026% copper (II) chloride (purity > 99%, Merck) solution, and the pH of the solution is maintained at 3.1 by adding required amount of glacial acetic acid (purity > 99%, Merck).
Nevertheless, low levels of these compounds could have been maintained at steady state, and significant amounts of NH3 could have dissolved in the oceans if the pH of the early oceans was lower than the pKa of NH3 (∼9.2 at 25 degrees Celsius) (Bada and Miller 1968).
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