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We find that to maintain a youthful cardiac and organismal physiology, the pathway's activity needs to be finely tuned and maintained at specific activity levels, and this set point appears to be altered or deregulated during aging.
Eight to twelve week old female 129Sv/Ev, C57BL/6, Il17a −/− and Il17ra −/− mice on C57BL/6 background were bred and maintained at specific pathogen-free facilities at the University of Aberdeen and University of Pittsburgh.
However, at endogenous pericentromeric repeat sequences, H3K9me and heterochromatin can still be maintained at specific sites by the action of the H3K9 methyltransferase Clr4 even when silencing of both strands is released by mutation of RNAi (Noma et al. 2004; Sadaie et al. 2004; Li et al. 2008).
The Na+ and Cl− concentrations in S. virginicus cultured cells were maintained at specific levels in the 300 and 500 mM NaCl treatments, while those in rice cultured cells increased together with an increasing NaCl concentration in the medium, especially under 500 mM NaCl (Fig. 5F and H).
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During constant current charging, the charging current is maintained at a specific level until the state of charge (SOC) of the battery reaches a certain level.
At pre-fixed filtration time of 5 min, continuous operation at supra-critical fluxes (47.1 56.8 L/h m2) was maintained at low specific aeration demands (SADpnet= 6.1 8.2 Nm3air/m3permeate).
The solutions were dispersed with supercritical CO2, acting as an anti-solvent, through a specially designed co-axial nozzle into a pressured vessel maintained at a specific constant temperature and pressure.
Mice were maintained at the Specific Pathogen-Free (SPF) unit, Faculty of Medicine, Hebrew University, under a 12 h light/dark cycle, and provided with food and water ad libitum.
Wild-type C. elegans and C. briggsae strains were maintained at strain-specific optimal temperatures on nematode growth media (NGM) agar plates seeded with E. coli OP50 as described by Brenner [25].
Only when this radiation is maintained at a highly specific frequency will it induce the quantum transition (energy change) of the cesium or rubidium atoms.
Yet, whether the initial separation of both types of information is maintained at higher language-specific processing levels has to be figured out.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com