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Cell cultures were maintained at routine incubation settings (37°C, 5% CO2) and optimum humidity.
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Total DNA was extracted from pure cultures of Phytophthora according to Schena and Cooke, diluted to 10 ng/μl and maintained at 5°C for routine amplifications and at -20°C for long term storage [ 42].
All strains were routinely maintained at -80°C in 15% glycerol for long-term storage and at 4°C in silica gel [ 33] for routine use.
Routine cultures were maintained at 24 + 1°C, 60% RH, and 12 hrs light (9 AM to 9 PM) and 12 hrs dark cycle.
In view of our findings and other evidence available, the routine of continuing anti-TNF treatment perioperatively is maintained at our center.
Resolution was maintained at high flow rates (up to 900 cm/h) and utilizing shorter gradient development routines.
Both routine and experimental medium was replaced every 4 days and the cultures were maintained at 37°C in a humidified 5% CO2 atmosphere.
Routine and total metabolism increased exponentially with temperature, while apparent heat increment (RAHI) was higher for octopuses maintained at 18 °C than in the rest of the treatments.
The effects were maintained at follow-up.
Gain was always maintained at 1.0x.
The culture was maintained at 2% hematocrit.
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