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At external pH 6, the initial cytoplasmic pH was significantly lower than the cytoplasmic pH maintained at external pH 7 (see Fig. 3A), but the cells still maintained substantial pH homeostasis, with a full unit of ΔpH (Fig. 3D).
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In contrast, during the growth of Geobacter sulfurreducens, the medium was maintained at neutral pH without external addition of either acid or base.
Our results suggest that even under a hypothetical scenario in which SCS and CAS are maintained at constant values by external intervention and their influence on variability is nullified, the firming process is expected to vary, with part of the variability being attributable to the additive genetic component.
The cochleae were dissected in either external solution maintained at 4°C and containing (in mM): 5.8 KCl; 144 NaCl; 0.9 MgCl2; 1.3 CaCl2; 0.7 NaHEPES; 10 HEPES and 5 glucose (pH 7.4, ∼300 mOsm) for electrophysiological recordings or phosphate buffered saline maintained at 4°C for immunofluorescence experiments.
Animal temperature was maintained at 38.0 ± 1.5°C using external warming devices and warmed fluids.
Consequently, such inland fisheries fall more within the agricultural definition of sustainability, whereby sustainability is a function of the inputs and outputs that can be maintained at no cost to the external environment.
The temperature inside the incubator was maintained at a stable 28°C using external air heating device for both the control and the exposed samples throughout the whole duration of the experiment.
Temperature and humidity measurements were taken inside the shelter while the external temperature was maintained at −20 °C.
The model predicted antagonistic co-contraction must increase with potential energy of the system even when the external moment was maintained at a constant value.
Although there are no detailed studies on the pH of fungal nuclei, it is known that the cytoplasmic pH of Anidu is maintained at 7.6 under a variety of external conditions [59].
Core body temperature was maintained at 35 ± 0.5°C using an external temperature controlled heat lamp.
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