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Cell cultures were maintained at densities between 1 × 10 and 1 × 10 cells/ml.
The cells were stored in liquid nitrogen since 1990 and, when cultured, were maintained at densities below 5 × 10/ml.
Larvae were maintained at densities of approx. 150 per 2 L container and fed finely ground fish food (Tetramin, Melle, Germany).
Adults were typically maintained at densities of 600 800 adults per enclosure and provided with water and a 5% glucose solution ad libitum.
Cell cultures were maintained at densities between 1 × 10 and 1 × 10 cells/ml and harvested by centrifugation when cultures reached ∼8 × 10 cells/ml.
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All species were maintained at culture densities between 1 × 105 and 1 × 107 cells/ml by repeated subculture, culture density was measured with a CASY model TT cell counter (Roche Diagnostics, Burgess Hill, UK).
Where the hills aren't burnt for grouse they are grazed to the roots by overstocked deer, maintained at vast densities to give the bankers waddling over the moors in tweed pantaloons a chance of shooting one.
Cells were maintained at low densities (75% confluence) and passaged until reaching the confluent state, usually every 3 4 days on 90 mm cell culture dishes.
During development, larvae were maintained at similar densities and with ad libitum access to food and water.
We then test the primary prediction of the model – that host range should be negatively correlated with host abundance – by examining the ecological and evolutionary dynamics of phage populations in laboratory microcosms maintained at host densities above and below this threshold.
Even if a farm's livestock is wiped out by an infection, new animals may be brought in and maintained at high densities again.
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